Validation of housekeeping genes for normalizing RNA expression in real-time PCR
نویسندگان
چکیده
منابع مشابه
Validation of housekeeping genes for normalizing RNA expression in real-time PCR.
Analysis of RNA expression using techniques like real-time PCR has traditionally used reference or housekeeping genes to control for error between samples. This practice is being questioned as it becomes increasingly clear that some housekeeping genes may vary considerably in certain biological samples. We used real-time reverse transcription PCR (RT-PCR) to assess the levels of 13 housekeeping...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2004
ISSN: 0736-6205,1940-9818
DOI: 10.2144/04371rr03